A vitamin-K2-binding factor secreted from Bacillus subtilis
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چکیده
منابع مشابه
Enhanced Production of Vitamin K2 from Bacillus subtilis (natto) by Mutation and Optimization of the Fermentation Medium
The aim of this study was to enhance the production of vitamin K2 by using N-methyl-N-nitro-N-nitroso-guanidine (NTG) and low energy ion beam implantation and optimizing the fermentation medium. Mutation resulted in 1.66fold higher production of vitamin K2 than that of the parentl strain. The production by the mutant BN-P15-11-1 was increased 55% and reached 3.593±0.107 mg/L by using the Placke...
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AppA is the membrane-anchored extracellular receptor component of an ABC transporter responsible for the uptake of oligopeptides into Bacillus subtilis. AppA has been overexpressed as a cleavable maltose-binding protein fusion in Escherichia coli. Following removal of the fusion portion, AppA has been crystallized from morpholinoethanesulfonic acid-buffered solutions at pH 6.5 containing polyet...
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The hyperthermophilic α-amylase from Thermococcus sp. HJ21 possesses unique traits (Ca(2+)-independent thermostability and optimal temperature of 95°C) that make it a great potential candidate for use in the food industry. However, this Archaea isolated from a deep-sea thermal vent requires strict control of culture conditions and produces only small amounts of α-amylase. To solve these problem...
متن کاملSigma factor displacement from RNA polymerase during Bacillus subtilis sporulation.
As Bacillus subtilis proceeds through sporulation, the principal vegetative cell sigma subunit (sigma(A)) persists in the cell but is replaced in the extractable RNA polymerase (RNAP) by sporulation-specific sigma factors. To explore how this holoenzyme changeover might occur, velocity centrifugation techniques were used in conjunction with Western blot analyses to monitor the associations of R...
متن کاملVitamin K2 Monograph
Introduction Vitamin K is a fat-soluble vitamin essential for the functioning of several proteins involved in blood clotting.1 Discovered in 1929 by Danish scientist Henrik Dam, the vitamin received the letter “K” because the initial discoveries were reported in a German journal in which the substance was designated as “Koagulationsvitamin.” Research during the last 30 years has resulted in gre...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1990
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1990.tb19218.x